Journal: Translational Cancer Research

Article Title: Long non-coding RNA (LncRNA) CHROMR promotes the expression of the CNNM1 gene by adsorbing hsa-miR-1299 to obtain drug resistance in diffuse large B lymphoma cells

doi: 10.21037/tcr-22-1087

Figure Lengend Snippet: CHROMR’s role in the DLBCL cell line. (A) After CHROMR overexpression, detect CHROMR, miR-1299, and CNNM1 expression changed in SU_DHL_4 cell line by RT-qPCR. (B) After CHROMR overexpression, we detected apoptosis-related genes and CNNM1 expression by Western Blot. (C) In the case of rituximab killing, flow cytometry detected the proportion of cells in the G2 phase of each group. (D) In the case of rituximab killing, the proportion of apoptosis in each group was detected by flow cytometry. (E) After CHROMER overexpression, we detected cell proliferation ability of each group by cck8. *, P<0.05; ***, P<0.001. NC, negative control; DLBCL, diffuse large B-cell lymphoma; RT-qPCR, real time quantitative polymerase chain reaction.

Article Snippet: When necessary, 17 nM rituximab (MCE) was added to the medium to induce cell death.

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry, Negative Control, Real-time Polymerase Chain Reaction

Journal: Translational Cancer Research

Article Title: Long non-coding RNA (LncRNA) CHROMR promotes the expression of the CNNM1 gene by adsorbing hsa-miR-1299 to obtain drug resistance in diffuse large B lymphoma cells

doi: 10.21037/tcr-22-1087

Figure Lengend Snippet: Verification of the CHROMR/miR-1299/CNNM1 pathway through cell function, RT-qPCR, and Western Blot experiments. (A) After transfection of CHROMR-over plasmid and miR-1299 mimic recovery, we detected CHROMR, miR-1299, CNNM1 expression changes in SU_DHL_4 cell line by RT-qPCR. (B) After transfection of CHROMR-over plasmid and miR-1299 mimic recovery, we detected apoptosis-related genes and CNNM1 expression by Western Blot. + indicates that the substance is transfected, − indicates that the substance is not transfected. (C) In the case of rituximab killing, flow cytometry detected the proportion of cells in the G2 phase of each group. (D) In the case of rituximab killing, the proportion of apoptosis in each group was detected by flow cytometry. ***, P<0.001. NC, negative control; RT-qPCR, real time quantitative polymerase chain reaction.

Article Snippet: When necessary, 17 nM rituximab (MCE) was added to the medium to induce cell death.

Techniques: Cell Function Assay, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Expressing, Flow Cytometry, Negative Control, Real-time Polymerase Chain Reaction

Journal: Bioengineering

Article Title: Growth Behavior of Human Adipose Tissue-Derived Stromal/Stem Cells at Small Scale: Numerical and Experimental Investigations

doi: 10.3390/bioengineering5040106

Figure Lengend Snippet: Results of flow cytometric measurements. The results show the percentage of positive cells for a given surface marker. A one-way ANOVA (Holm–Sidak method, n = 3; p < 0.05) with multiple comparisons versus the control group was performed for the statistical analysis.

Article Snippet: For flow cytometric analysis, the cells were stained with fluorochrome-conjugated anti-human CD14, CD20, CD34, CD45, CD73, CD90, and CD105 (according to the recommendations of the International Society for Cellular Therapy ISCT and the International Federation for Adipose Therapeutics and Science IFATS) antibodies (Miltenyi Biotec, Germany) and measured with a MACSQuant device.

Techniques: Marker, Control